anti integrinβ1 antibody  (Santa Cruz Biotechnology)

Journal: Micromachines

Article Title: A Microfluidic-Based Cell-Stretching Culture Device That Allows for Easy Preparation of Slides for Observation with High-Magnification Objective Lenses

doi: 10.3390/mi16010093

Figure Lengend Snippet: Effects of cyclic stretching on integrinβ1 and vinculin localization in OP9 cells. Fluorescence images of integrinβ1 (green), f-actin (red), and vinculin (gray) in OP9 cells cultured on the device after 72 h under static conditions followed by 24 h under ( A ) static or ( B ) cyclic stretching conditions (0.5 Hz) photographed with a 100× oil immersion objective lens. Confocal images were captured every 0.48 µm along the z-axis to create z-stacks (14 slices) for maximum-intensity projection (scanning zoom 2×). The right figure shows the fluorescence intensity–distance profiles of integrinβ1 (green), f-actin (red), vinculin (gray), and nuclei (blue) on lines ( a – d ) drawn on the microscope image (MIP).

Article Snippet: For integrinβ1, vinculin, f-actin, and nuclear staining, 0.5% BSA was added with anti-integrinβ1 antibody (0.4 μg/mL; SC-19656, Santa Cruz Biotechnology, Dallas, TX, USA), anti-vinculin antibody (4 μg/mL; 26520-1-AP, Proteintech, Rosemont, IL, USA), and rhodamine phalloidin (1.65 μM; R4159, Thermo Fisher Scientific) and incubated overnight at 4 °C.

Techniques: Fluorescence, Cell Culture, Microscopy

Journal: iScience

Article Title: Exosomal Galectin-3 promotes peritoneal metastases in gastric adenocarcinoma via microenvironment alterations

doi: 10.1016/j.isci.2024.111564

Figure Lengend Snippet:

Article Snippet: Integrinβ1 , Santa Cruz , Cat# sc-8978, RRID: AB_2130101.

Techniques: Microarray, Recombinant, Reverse Transcription, SYBR Green Assay, Enzyme-linked Immunosorbent Assay, Staining, Plasmid Preparation, Bicinchoninic Acid Protein Assay, Software